Journal: International Journal of Molecular Sciences

Article Title: The Effect of Isosaponarin Derived from Wasabi Leaves on Glutamate Release in Rat Synaptosomes and Its Underlying Mechanism

doi: 10.3390/ijms23158752

Figure Lengend Snippet: Isosaponarin decreases 4-AP-evoked phosphorylation of SNAP-25 and MARCKS. Upper panel, Western blot bands of SNAP-25, pSNAP-25 (Ser187), pMARCKS (Ser152/156), and β-actin. Lower panel, expressions of SNAP-25, pSNAP-25 (Ser187), and pMARCKS were normalized with β-actin. Isosaponarin was added 10 min before the addition of 4-AP. Data are presented as mean ± SEM (n = 5 per group). *** p < 0.001 vs. control group; # p < 0.001 vs. 4-AP-treated group.

Article Snippet: The primary antibodies used were PKC (1:600, Abcam, Cambridge, UK); pPKC (1:1000; Cell Signaling, Beverly, MA, USA); PKCα (1:600; Cell Signaling, Beverly, MA, USA); pPKCα (1:2000; Abcam, Cambridge, UK); SNAP-25 (1:20,000; Abcam, Cambridge, UK); pSNAP-25 (Ser187) (1:2000; Abcam, Cambridge, UK); pMARCKS (Ser152/156) (1:250; Cell Signaling, Beverly, MA, USA); and β-actin (1:1000; Cell Signaling, Beverly, MA, USA).

Techniques: Phospho-proteomics, Western Blot, Control